Direct PCR Reagents and Kits
Clontech Labs 3P TaKaRa Ex Taq® DNA Polymerase, 3,000 units
TaKaRa Ex Taq DNA Polymerase combines the proven performance of Takara Taq polymerase with the proofreading activity of an efficient 3'-to-5' exonuclease, for high-sensitivity, high-efficiency PCR. Ex Taq can also be used for long-range PCR (up to 20 kb from genomic DNA templates and up to 30 kb from lambda DNA templates). Separate tubes of Mg2+ plus buffer and dNTP mix are supplied with the polymerase. 3,000 units
Apexbio Technology LLC 1STEP COMP CELL PREP KIT 200T
1STEP COMP CELL PREP KIT 200T APEXBIO TECHNOLOGIES
Sigma Aldrich Fine Chemicals Biosciences Diffinity RapidTipR for PC
Diffinity RapidTip effectively removes dNTPs primers and primer dimers while providing greater than 90% recovery of pure DNA fragments from 100 bp to 10 kb. The functional pipette tip contains everything you need for PCR purification. The tip is filled with a proprietary adsorption technology that has a differential affinity for PCR components. The dispensed SOLUTIONon yields purified high quality DNA ready for downstream applications such as DNA sequencing SNP analysis and microarray printing.
Clontech Labs 3P Advantage® 2 Polymerase Mix, 100 Rxns
Advantage 2 Polymerase Mix is a hot-start PCR polymerase mix that contains Takara's Titanium Taq DNA Polymerase (with TaqStart Antibody), and a minor amount of a proofreading polymerase for a balance of higher yield and higher fidelity than wild-type Taq. The Mix is ideal for amplification of long templates up to 18 kb and complex genomic DNA up to 6 kb. Two different optimized buffers are supplied with the polymerase mix. dNTPs need to be purchased separately. Enough enzyme mix and PCR buffer are supplied for 100 PCR reactions of 50 μl each.
Roche Diagnostics HIGH PURE PCR TEMPLT PREP KIT
High Pure PCR Template Preparation Kit, 1 kit; product cross to QiAGEN Dneasy blood and tissue kit
Bio Rad Laboratories QX200 ddPCR EvaGreen Supermix, 2x concentrated, 5 ml (5 x 1 ml vials), 500 x 20 μl reactions
QX200 ddPCR EvaGreen Supermix, 2x concentrated, 5 ml (5 x 1 ml vials), 500 x 20 μl reactions
New England Biolabs, Inc. Taq 5X Master Mix – 500 reactions (50 µl vol)
Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5' to 3' polymerase activity and a 5' flap endonuclease activity. Taq 5X Master Mix is an optimized ready-to-use solution containing Taq DNA Polymerase, dNTPs, MgCl2, KCl and stabilizers. It is best for routine PCR applications from templates including pure DNA solutions, bacterial colonies, and cDNA. It can amplify up to 4 kb from complex genomic DNA or up to 5 kb from lambda DNA.
Viagen Biotech Inc DIR PCR LYSIS RGNT(MOUSE TAIL
Viagen DirectPCR� DNA Extraction System is a single-tube system for rapid preparation of DNA from mouse tails, ear pieces, yolk sac, and culture cells. The patent-pending components developed by scientists at Viagen Biotech Inc. allow the resulting DNA extracts to be compatible with genomic PCR for genotyping. DirectPCR� system offers advantages over conventional protocols that include: � Time saving: Less hands-on time. Crude tail lysates for PCR. � Money saving: Cost-effective reactions. � Safe: No organic reagents. � Environmental: Less waste (organic reagents, tubes, tips, etc...) � Reliable and efficient: Virtually 100% success rate with high yields. 50ml (250 mouse tails).
Reprocell STEMOLECULE PD0325901 2 MG
PD0325901 is a small molecule targeting mitogen-activated protein kinase (MaPK/ERK kinase or MEK) with potential antineoplastic activity. PD0325901, a derivative of MEK inhibitor CI-1040, selectively binds to and inhibits MEK, which may result in the inhibition of the phosphorylation and activation of MaPK/ERK and the inhibition of tumor cell proliferation1,2. along with the aLK5 inhibitor SB431542, PD0325901 has also been shown to increase the efficiency of reprogramming human primary fibroblasts into induced pluripotent stem (iPS) cells3. 2mg.
Sigma Aldrich Fine Chemicals Biosciences ReadyMixTM Taq PCR Reactio
ReadyMix(TM) Taq PCR Reaction Mix is a prepared SOLUTIONon containing everything needed for a PCR reaction except the specific primers and template. The mix includes Sigmas high quality Taq DNA Polymerase 99% pure deoxynucleotides and buffer in a 2x optimized reaction concentrate. for reaction set-up add the ReadyMix (25 muL) to the primers template and water (total volume 50 muL). Using ReadyMix Taq PCR Reaction Mix reduces pipetting steps and risk of contamination. This saves time and reduces errors while still providing the great performance of Sigmas Taq DNA Polymerase.