Direct PCR Reagents and Kits
Selleck Chemical LLC Mouse Direct PCR Kit
The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication
Roche Diagnostics LIGHTCYCLER FS DNA MAST HYB
LightCycler FastStart DNA Master PLUS HybProbe - 1 kit (for 96 reactions of 20 uL final reaction volume)
Roche Diagnostics LIGHTCYCLER FASTSTART DN
LightCycler FastStart DNA Master HybProbe - 1 kit (for 480 reactions of 20 uL final reaction volume)
Roche Diagnostics LIGHTCYCLER CONTROL KIT
LightCycler Control Kit DNA - 1 kit (for 50 reactions of 20 uL final reaction volume)
New England Biolabs, Inc. Taq 5X Master Mix – 500 reactions (50 µl vol)
Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5' to 3' polymerase activity and a 5' flap endonuclease activity. Taq 5X Master Mix is an optimized ready-to-use solution containing Taq DNA Polymerase, dNTPs, MgCl2, KCl and stabilizers. It is best for routine PCR applications from templates including pure DNA solutions, bacterial colonies, and cDNA. It can amplify up to 4 kb from complex genomic DNA or up to 5 kb from lambda DNA.
Clontech Labs 3P PrimeSTAR® HS DNA Polymerase (premix), 100 Rxns
A 2X hot-start PCR master mix containing Takara's high-fidelity PrimeSTAR HS DNA polymerase, optimized reaction buffer, and dNTPs. PrimeSTAR HS DNA polymerase has superior proofreading ability due to robust 3' to 5' exonuclease activity. It can efficiently amplify up to 8.5 kb for human genomic DNA targets or up to 22 kb for lambda DNA. Please refer to the User Manual for premix composition. 100 Rxns
Molecular Research Center DNAzol Direct, 25 mL
DNAzol Direct is a universal reagent for processing biological samples for direct PCR. No DNA isolation is required. DNAzol Direct is designed to process a wide range of samples including animal, plant, fungi, yeast, bacterial, and viral samples. It has been used with blood, serum and plasma, saliva, buccal swabs, blood cards, hairs and feathers, leaves and seeds, and formalin-fixed tissues.
Reprocell STEMOLECULE PD0325901 2 MG
PD0325901 is a small molecule targeting mitogen-activated protein kinase (MaPK/ERK kinase or MEK) with potential antineoplastic activity. PD0325901, a derivative of MEK inhibitor CI-1040, selectively binds to and inhibits MEK, which may result in the inhibition of the phosphorylation and activation of MaPK/ERK and the inhibition of tumor cell proliferation1,2. along with the aLK5 inhibitor SB431542, PD0325901 has also been shown to increase the efficiency of reprogramming human primary fibroblasts into induced pluripotent stem (iPS) cells3. 2mg.
Boca Scientific Inc Maxime PCR PreMix Kit (i-StarTaq™), HotStart, 96 Tubes
Maxime PCR PreMix Kit (i-StarTaq™), HotStart contains all components necessary for a PCR reaction in a dried and stable format in each tube. PCR can be carried out immediately with the addition of the template, primer and distilled water, reducing the risk of pipetting error. Gel loading electrophoresis is also possible as the tubes contain loading dye. This kit features Intron’s i-StarTaq™ DNA Polymerase. This polymerase implements a chemical-based HotStart function that is not antibody-based and improves specificity and accuracy. This kit has excellent stability due to oxidation and moisture prevention achieved by a vacuum compression manufacturing method. Each tube contains i-StarTaq™ DNA Polymerase, dNTPs, reaction buffer(10x), and gel loading buffer.
Clontech Labs 3P Advantage® 2 Polymerase Mix, 100 Rxns
Advantage 2 Polymerase Mix is a hot-start PCR polymerase mix that contains Takara's Titanium Taq DNA Polymerase (with TaqStart Antibody), and a minor amount of a proofreading polymerase for a balance of higher yield and higher fidelity than wild-type Taq. The Mix is ideal for amplification of long templates up to 18 kb and complex genomic DNA up to 6 kb. Two different optimized buffers are supplied with the polymerase mix. dNTPs need to be purchased separately. Enough enzyme mix and PCR buffer are supplied for 100 PCR reactions of 50 μl each.